紫外交聯(lián)儀在實驗中的具體應(yīng)用有哪些？

更新時間：2022-09-01 點擊次數(shù)：1126次

紫外交聯(lián)儀的應(yīng)用/ UV crosslinker APPLICATIONS

紫外交聯(lián)儀UCL-3200/3500 也可以在需要計量紫外線強度時使用。短波 (254nm)、中波 (312nm) 或長波 (365nm) 紫外管均可用于此類應(yīng)用。在 INTENSITY 模式下，該裝置還可用于監(jiān)測室內(nèi)的強度。然而，在這種模式下，燈一直亮著，直到按下“小數(shù)點"按鈕。

The UCL-3200/3500 can also be used whenever a metered UV dosage is required. Either short wave (254nm), medium wave (312nm), or long wave (365nm) UV tubes may be used for such applications. In the INTENSITY mode, the unit may also be used to monitor intensity inside the chamber. In this mode, however, the lamps remain on until either the "."’ button is pressed or the power is turned off。

常用于切割 DNA/ NICKING DNA；基因圖譜/GENE MAPPING；測試和篩選 RecA TESTING AND SCREENING RecA；紫外線殺菌/UV STERILIZATION；消除 PCR* 污染物/ELIMINATING PCR* CONTAMINANTS

紫外交聯(lián)儀UCL-3200/3500 可用于在 Northern、Southern、狹縫或斑點印跡以及菌落或斑塊剝離后將核酸共價結(jié)合到膜上。研究表明，120 mJ/cm2 是在任何這些程序中將 DNA 或 RNA 附著到尼龍或硝酸纖維素膜上的佳劑量。為了在 DNA 或 RNA 轉(zhuǎn)移步驟后獲得佳結(jié)果，應(yīng)在膜干燥后將膜放入 UCL-3200/3500 室內(nèi)。放置膜，使核酸附著的一面朝上（即朝向光源），以便直接照射核酸。選擇一個能量值，然后按“START"以啟動所需功能。

The UCL-3200/3500 can be used to covalently bind nucleic acids to membranes after Northern, Southern, slot or dot blotting, and colony or plaque lifts. Studies indicate that 120 mJ/cm2 is an optimal dosage for attachment of DNA or RNA in any of these procedures to nylon or nitrocellulose membranes. In order to obtain the best results following the DNA or RNA transfer step, the membrane should be placed inside the UCL-3200/3500 chamber after the membrane is dried. Place the membrane with the side to which the nucleic acids are attached facing upwards (i.e. towards the light source) so that the nucleic acids are directly irradiated. Select an energy value followed by ‘‘START’’ to initiate the desired function.

紫外交聯(lián)儀UCL-3200/3500操作面板

切割 DNA/ NICKING DNA

紫外交聯(lián)儀UCL-3200/3500 可用于在瓊脂糖凝膠中切割溴化乙錠染色的 DNA，以代替脫嘌呤洗滌液。

The UCL-3200/3500 can be used to nick the ethidium-bromide-stained DNA in agarose gels in place of a depurination wash.

基因圖譜/GENE MAPPING

紫外交聯(lián)儀UCL-3200/3500 可用于在用限制酶處理之前產(chǎn)生胸腺嘧啶二聚體。在識別位點處或附近形成二聚體會抑制切割。

The UCL-3200/3500 can be used to create thymine dimers prior to treatment with a restriction enzyme. The formation of dimers at or near the recognition site inhibits cleavage 。

測試和篩選 RecA TESTING AND SCREENING RecA

紫外交聯(lián)儀UCL-3200/3500 可用于檢測和篩選 RecA 突變，該突變可以防止紫外線引起的細胞損傷修復(fù)，從而延緩其生長。

The UCL-3200/3500 can be used for testing and screening RecA mutation which can prevent repair of UV-induced damage on cells, thereby retarding their growth.